The complete breakdown and reformation of the nuclear envelope during cell division is one of the most dramatic structural and functional changes in higher eukaryotic cells. The overall aim of the Ellenberg group is to elucidate the mechanisms underlying mitotic nuclear remodeling in intact cells. We are using quantitative confocal microscopy to directly observe key proteins of these dynamic processes - fluorescently tagged with GFP and other fluorophores - in real time in live cells.
More details here.

- Genome-wide RNAi screens in human cells by automated microscopy to identify genes required for mitosis.

- Localization of mitotic gene products in human cells during cell cycle.

- Development of a fully integrated confocal screening system for cell based assays in human cells.

WP2, WP3, WP1

Jan Ellenberg
Gene Expression and Cell Biology/Biophysics Programmes
European Molecular Biology Laboratory
Meyerhofstrasse 1,
D-69117 Heidelberg
Germany

Email: Jan.Ellenberg@embl-heidelberg.de